FRESHNEY EBOOK PDF

FRESHNEY EBOOK PDF

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June 15, 2020

R. Ian Freshney. First published:9 March Print ISBN | Online ISBN |DOI/ Copyright © . This masterful third edition of Freshney’s Culture of Animal Cells updates and considerably expands the scope of its predecessor and still enables both the. A Manual of Basic Technique. Author, R. Ian Freshney. Edition, 4, illustrated. Publisher, Wiley, ISBN, , Length, pages.

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Enviado por Larissa flag Denunciar. Terminal ductal lobular-like unit cultured from normal human mammary epithelium [Labarge et al. Nestin expression in replated neurospheres from human ES cells [Jackson et al. While the publisher and author have used their best efforts in preparing this book, they make no representations or warranties with respect to the accuracy or completeness of the contents of this book and specifically disclaim any implied warranties of merchantability or fitness for a particular purpose.

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Culture of Animal Cells – R. Ian Freshney – Google Books

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For more information about Wiley products, visit our web site at www. Library of Congress Cataloging-in-Publication Data: Culture of animal cells: ISBN cloth 1. Historical Background, 1 1. Advantages of Tissue Culture, 6 1. Control of the Environment, 6 1. Characterization and Homogeneity of Samples, 6 1. Economy, Scale, and Mechanization, 6 1. In vitro Modeling of In vivo Conditions, 7 1. Dedifferentiation and Selection, 8 1. Origin of Cells, 8 1. Major Differences In vitro, 8 1. Ffreshney of Tissue Culture, 8 2.

Freshneey of Cultured Cells, 11 2. The Culture Environment, freshey 2. Cell Adhesion, 11 2. Cell Adhesion Molecules, 11 2. Intercellular Junctions, 12 2.

Extracellular Matrix, 13 2. Cell Motility, 14 2. Cell Proliferation, 15 2. Cell Cycle, 15 2. Control of Cell Proliferation, 15 2. Maintenance of Differentiation, 17 2. Cell Signaling, 17 2. Energy Metabolism, 19 2. Origin of Cultured Cells, 20 2. Initiation of the Culture, 21 2. Evolution of Cell Lines, 21 2. Transformation and the Development of Continuous Cell Lines, 22 3. Laboratory Design, Layout, and Equipment, 25 3.

Layout, Furnishing, and Services, 25 3. Sterile Handling Area, 30 3. Laminar Flow, 30 3. Service Bench, 30 3. Preparation Area, 33 3. Equipment and Materials, 37 4. Requirements of a Tissue Culture Laboratory, 37 4.

Aseptic Area, 37 4. Laminar-Flow Hood, 37 4. Service Carts, 41 4. Sterile Liquid Handling—Pipetting and Dispensing, 41 4. Inverted Microscope, 45 4. CCD Camera and Monitor, 46 4.

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Dissecting Microscope, 46 4. Cell Counting, 47 4. Incubation and Culture, 47 4. Humid CO2 Incubator, 48 4. Temperature Recorder, 48 4. Roller Racks, 49 4. Eblok Stirrer, 50 4. Culture Vessels, 50 4. Preparation and Sterilization, 50 4. Preparation of Media and Reagents, 51 4.

Refrigerators and Freezers, 54 4. Cryostorage Containers, 55 4. Controlled-Rate Freezer, 55 4. Supplementary Laboratory Equipment, 55 4. Computers and Networks, 55 4. Upright Microscope, 55 4. Low-Temperature Freezer, 56 4. Confocal Microscope, 56 4. PCR Thermal Cycler, 56 4. Specialized Equipment, 56 4.

Culture of Animal Cells eBook by R. Ian Freshney

Microinjection Facilities, 56 4. Colony Counter, 56 4. Centrifugal Elutriator, 56 4. Flow Cytometer, 56 5. Aseptic Technique, 57 5. Objectives of Aseptic Technique, 57 5.

Risk of Contamination, 57 5. Maintaining Sterility, 57 5.

R. Ian Freshney

Elements of Aseptic Environment, 58 5. Laminar Flow, 58 5. Quiet Area, 60 5. Work Surface, 61 5. Personal Hygiene, 61 5.

Culture of Animal Cells by R. Ian Freshney (ebook)

Reagents and Media, 61 5. Sterile Handling, 61 5. Handling Bottles and Flasks, 64 5. Standard Procedure, 65 Protocol 5.

Working on the Open Bench, 67 Protocol 5. Handling Dishes or Plates, 69 5. Apparatus and Equipment, 69 5.

Boxed Cultures, 70 5. Gassing with CO2, 70 6. Safety, Bioethics, and Validation, 71 6.